Coding

Part:BBa_K2557007:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TP901 recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 633
    Illegal PstI site found at 1344
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 633
    Illegal PstI site found at 1344
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
    Illegal BamHI site found at 1599
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 633
    Illegal PstI site found at 1344
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 633
    Illegal PstI site found at 1344
    Illegal NgoMIV site found at 99
    Illegal NgoMIV site found at 243
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 852


Design Notes

Since we used animal cells as the chassis organism, the coding sequence was humanized.


Source

We found the coding sequence of TP901 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References